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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIGK Recombinant antibody

PIGK Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241989D4

Cat no : 84568-3-RR

Synonyms

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIGK Recombinant antibody (84568-3-RR)

    WB analysis using 84568-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84568-3-RR (PIGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84568-3-RR

    Affinity and Kinetic Characterization of 84568-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84568-3-RR against Human PIGK were performed. The affinity constant is 0.501 nM.

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Tested Applications

Positive WB detected inA549 cells, K-562 cells, HeLa cells, HT-1080 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84568-3-RR targets PIGK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIGK fusion protein Ag3497 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class K
Calculated molecular weight 45 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC026186
Gene symbol PIGK
Gene ID (NCBI) 10026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

Protocols

Product Specific Protocols
WB protocol for PIGK antibody 84568-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols