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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIGM Polyclonal antibody

PIGM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20421-1-AP

Synonyms

PIG-M, PIG M, Phosphatidylinositol-glycan biosynthesis class M protein, GPI-MT-I, GPI mannosyltransferase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIGM Polyclonal antibody (20421-1-AP)

    WB analysis of HeLa using 20421-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20421-1-AP (PIGM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PIGM Polyclonal antibody (20421-1-AP)

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PIGM Polyclonal antibody (20421-1-AP)

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIGM Polyclonal antibody (20421-1-AP)

    IHC staining of human kidney using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIGM Polyclonal antibody (20421-1-AP)

    IHC staining of human kidney using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIGM Polyclonal antibody (20421-1-AP)

    IF Staining of HeLa using 20421-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20421-1-AP (PIGM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20421-1-AP targets PIGM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGM fusion protein Ag14245 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class M
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC019865
Gene symbol PIGM
Gene ID (NCBI) 93183
RRIDAB_10734320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIGM antibody 20421-1-APDownload protocol
IHC protocol for PIGM antibody 20421-1-APDownload protocol
IF protocol for PIGM antibody 20421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols