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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIGO Polyclonal antibody

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16369-1-AP

Synonyms

UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIGO Polyclonal antibody (16369-1-AP)

    WB analysis of HeLa using 16369-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PIGO Polyclonal antibody (16369-1-AP)

    WB analysis of COLO 320 using 16369-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PIGO Polyclonal antibody (16369-1-AP)

    WB analysis of A431 using 16369-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using PIGO Polyclonal antibody (16369-1-AP)

    WB analysis of mouse spleen using 16369-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human liver using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human spleen using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human kidney using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human kidney using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human testis using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human testis using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human skin using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human skin using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PIGO Polyclonal antibody (16369-1-AP)

    IHC staining of human spleen using 16369-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PIGO Polyclonal antibody (16369-1-AP)

    IF Staining of MCF-7 using 16369-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, COLO 320 cells, mouse spleen tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16369-1-AP targets PIGO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGO fusion protein Ag9232 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol glycan anchor biosynthesis, class O
Calculated molecular weight1089aa,119 kDa; 454aa,50 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC013987
Gene symbol PIGO
Gene ID (NCBI) 84720
RRIDAB_2164806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIGO antibody 16369-1-APDownload protocol
IHC protocol for PIGO antibody 16369-1-APDownload protocol
IF protocol for PIGO antibody 16369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols