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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIH1D1 Polyclonal antibody, PBS Only

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19427-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of human testis using 19427-1-AP (same clone as 19427-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of mouse eye using 19427-1-AP (same clone as 19427-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human uterus tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    WB analysis of human uterus using 19427-1-AP (same clone as 19427-1-PBS)

    human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PIH1D1 Polyclonal antibody (19427-1-AP)

    IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using PIH1D1 Polyclonal antibody (19427-1-AP)

    IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PIH1D1 Polyclonal antibody (19427-1-AP)

    IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19427-1-PBS targets PIH1D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIH1D1 fusion protein Ag13759 相同性解析による交差性が予測される生物種
Full Name PIH1 domain containing 1
Calculated molecular weight 290 aa, 32 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC001108
Gene symbol PIH1D1
Gene ID (NCBI) 55011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.