Featured Product
KD/KO Validated

PIK3C2A Polyclonal antibody

Name: PIK3C2A Polyclonal antibody
Brand: Proteintech
SKU: 22028-1-AP
Rating: 5.00 (1 reviews)

PIK3C2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 22028-1-AP

Synonyms

CPK, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 22028-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22028-1-AP
WB result of PIK3C2A antibody (22028-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIK3C2A transfected HeLa cells.
WB analysis of PC-3 using 22028-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22028-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 22028-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 22028-1-AP
IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:15000) with PC-3 cells lysate 1480 ug.
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 22028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 22028-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PIK3C2A antibody (22028-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

-/ -

Cat No. 22028-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, PC-3 cells, human placenta tissue
Positive IP detected in	PC-3 cells
Positive IHC detected in	mouse ovary tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 5 publications below

Product Information

22028-1-AP targets PIK3C2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIK3C2A fusion protein Ag16894 相同性解析による交差性が予測される生物種
Full Name	phosphoinositide-3-kinase, class 2, alpha polypeptide
Calculated molecular weight	1686 aa, 191 kDa
Observed molecular weight	191 kDa
GenBank accession number	BC113658
Gene symbol	PIK3C2A
Gene ID (NCBI)	5286
RRID	AB_11183760
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PIK3C2A antibody 22028-1-AP	Download protocol
IHC protocol for PIK3C2A antibody 22028-1-AP	Download protocol
IF protocol for PIK3C2A antibody 22028-1-AP	Download protocol
IP protocol for PIK3C2A antibody 22028-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Science PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation. Authors - Federico Gulluni KD Validated View Article
human	WB, IF	Cancer Cell Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function. Authors - Federico Gulluni View Article
rat	WB,IF	Cell Res Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation. Authors - Shu-Xin Zhang View Article
human	WB,IF	J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization Authors - Ellis L Ryan KD Validated View Article
human	IF, WB	J Cell Sci Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization. Authors - Ellis L Ryan View Article
human	WB	Front Cell Infect Microbiol The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection. Authors - Teng Li View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

HUAYI (Verified Customer) (01-17-2020)

This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.

Applications: Western Blot, Immunofluorescence,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: cancer cell lines

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIK3C2A Polyclonal antibody

PIK3C2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 22028-1-AP

WB analysis of HeLa using 22028-1-AP

WB analysis of PC-3 using 22028-1-AP

WB analysis of HeLa using 22028-1-AP

WB analysis of human placenta using 22028-1-AP

IP experiment of PC-3 using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IHC staining of mouse ovary using 22028-1-AP

IHC staining of mouse heart using 22028-1-AP

IHC staining of mouse heart using 22028-1-AP

IF Staining of HeLa using 22028-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Reviews