PI3 Kinase p110 Alpha Polyclonal antibody

Name: PI3 Kinase p110 Alpha Polyclonal antibody
Brand: Proteintech
SKU: 20583-1-AP

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20583-1-AP

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of HepG2 using 20583-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20583-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 20583-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 20583-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20583-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20583-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 20583-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 20583-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 20583-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 20583-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

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Cat No. 20583-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, COLO 320 cells, HeLa cells, A431 cells, mouse liver tissue
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:300-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 3 publications below

Product Information

20583-1-AP targets PI3 Kinase p110 Alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight	124 kDa
Observed molecular weight	120-130 kDa
GenBank accession number	NM_006218
Gene symbol	PIK3CA
Gene ID (NCBI)	5290
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 20583-1-AP	Download protocol
IHC protocol for PI3 Kinase p110 Alpha antibody 20583-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Oncogene KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer. Authors - H Zhang View Article
human	WB	Oncotarget Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma. Authors - Xiao-Qing Wang View Article
human	IHC	J Cancer Vasculogenic Mimicry in Prostate Cancer: The Roles of EphA2 and PI3K. Authors - Hua Wang View Article
mouse	WB	Mol Med Rep Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells Authors - Xinyan Pan View Article
	WB	Dev Comp Immunol Characterization, functional and signaling elucidation of pigeon (Columba livia) interferon-α: Knockdown p53 negatively modulates antiviral response. Authors - Siwen Li View Article
		Biomed Pharmacother Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells. Authors - Jing-Jing Mou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI3 Kinase p110 Alpha Polyclonal antibody

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 20583-1-AP

WB analysis of HeLa using 20583-1-AP

WB analysis of COLO 320 using 20583-1-AP

WB analysis of COLO 320 using 20583-1-AP

WB analysis of HeLa using 20583-1-AP

WB analysis of HeLa using 20583-1-AP

WB analysis of A431 using 20583-1-AP

WB analysis of mouse liver using 20583-1-AP

IHC staining of human cervical cancer using 20583-1-AP

IHC staining of human cervical cancer using 20583-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.

Vasculogenic Mimicry in Prostate Cancer: The Roles of EphA2 and PI3K.

Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells

Characterization, functional and signaling elucidation of pigeon (Columba livia) interferon-α: Knockdown p53 negatively modulates antiviral response.

Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.

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