Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, Jurkat cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 20 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
21890-1-AP targets PI3 Kinase p110 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PI3 Kinase p110 Alpha fusion protein Ag16586 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| Calculated molecular weight | 1068 aa, 124 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC113601 |
| Gene Symbol | PIK3CA |
| Gene ID (NCBI) | 5290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42336 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p110 Alpha antibody 21890-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p110 Alpha antibody 21890-1-AP | Download protocol |
| IF protocol for PI3 Kinase p110 Alpha antibody 21890-1-AP | Download protocol |
| IP protocol for PI3 Kinase p110 Alpha antibody 21890-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Funct l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways | ||
J Agric Food Chem β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway. | ||
J Hematol Oncol Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression. | ||
Sci Rep NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice. | ||
J Exp Clin Cancer Res LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway. | ||
Sci Rep Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines. |