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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    WB analysis of LNCaP using 67964-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    WB analysis of Jurkat using 67964-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67964-1-Ig (PIK3CD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using PI3 Kinase p110 Delta Monoclonal antibody (67964-1-Ig)

    IF Staining of LNCaP using 67964-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using PIK3CD antibody (67964-1-Ig, Clone: 2G11C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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PI3 Kinase p110 Delta Monoclonal antibody

PI3 Kinase p110 Delta Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67964-1-Ig
Clone No.2G11C6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

p110D, PI3 kinase subunit delta, PI3K delta, PIK3CD, PtdIns 3 kinase subunit delta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67964-1-Ig targets PI3 Kinase p110 Delta in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Delta fusion protein Ag26995 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, delta polypeptide
Calculated molecular weight 1044 aa, 120 kDa
Observed molecular weight110 kDa
GenBank accession numberBC132919
Gene Symbol PIK3CD
Gene ID (NCBI) 5293
RRIDAB_2918715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
IF protocol for PI3 Kinase p110 Delta antibody 67964-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols