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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIK3CG Polyclonal antibody (31229-1-AP)

    WB analysis using 31229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31229-1-AP (PIK3CG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PIK3CG Polyclonal antibody (31229-1-AP)

    IHC staining of human ovary cancer using 31229-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31229-1-AP (PIK3CG antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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PIK3CG Polyclonal antibody

PIK3CG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31229-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma, p120-PI3K, p120 PI3K, p110gamma, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31229-1-AP targets PIK3CG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIK3CG fusion protein Ag34951 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, gamma polypeptide
Calculated molecular weight 126 kDa
Observed molecular weight126 kDa
GenBank accession numberBC035683
Gene Symbol PIK3CG
Gene ID (NCBI) 5294
RRIDAB_3669910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP48736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PIK3CG antibody 31229-1-APDownload protocol
IHC protocol for PIK3CG antibody 31229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols