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  • Featured Product
  • KD/KO Validated

PIK3IP1 Polyclonal antibody, PBS Only

PIK3IP1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16826-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIK3IP1 Polyclonal antibody (16826-1-AP)

    WB analysis of HeLa using 16826-1-AP (same clone as 16826-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PIK3IP1 Polyclonal antibody (16826-1-AP)

    WB analysis of mouse liver using 16826-1-AP (same clone as 16826-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16826-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PIK3IP1 Polyclonal antibody (16826-1-AP)

    WB analysis of HepG2 using 16826-1-AP (same clone as 16826-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16826-1-AP (PIK3IP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16826-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PIK3IP1 Polyclonal antibody (16826-1-AP)

    IP experiment of HeLa using 16826-1-AP (same clone as 16826-1-PBS)

    IP result of anti-PIK3IP1 (IP:16826-1-AP, 4ug; Detection:16826-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16826-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16826-1-PBS targets PIK3IP1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIK3IP1 fusion protein Ag10427 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase interacting protein 1
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC011049
Gene symbol PIK3IP1
Gene ID (NCBI) 113791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3IP1(Phosphoinositide-3-kinase-interacting protein 1) is also named as HGFL. The class IA phosphoinositol-3-kinases (PI3Ks) regulate important cellular processes such as proliferation, growth, survival, motility and metabolism. PIK3IP1 is a transmembrane protein that possesses a region in its intracellular domain that shares homology with the p85 regulatory subunit of PI3K. It has 5 isoforms produced by alternative splicing with the MW of 11, 18, 19, 25,28 kDa. PIK3IP1 undergoes N- and O-linked amino acid glycosylation. The major glycosylated form of PIK3IP1 migrates at about 43 kDa in western blot analysis, while the unglycosylated form migrates at 37 kDa. It is also detected a 65 kDa variant in HepG2 cell lysate that PIK3IP1 is indeed membrane bound and that these variants arise from alternative splicing and/or post-translational processing events such as enzymatic proteolysis and/or glycosylation. (PMID:18632611).