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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI3 Kinase p85 Beta Recombinant antibody

PI3 Kinase p85 Beta Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240531B5

Cat no : 83606-5-RR

Synonyms

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIK3R2 Recombinant antibody (83606-5-RR)

    WB analysis using 83606-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PI3 Kinase p85 Beta Recombinant antibody (83606-5-RR)

    IF Staining of Jurkat using 83606-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PI3 Kinase p85 Beta Recombinant antibody (83606-5-RR)

    IF Staining of HeLa using 83606-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83606-5-RR is PI3 Kinase p85 Beta Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PI3 Kinase p85 Beta fusion protein Ag14367 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Calculated molecular weight 728 aa, 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC014170
Gene symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p85 Beta antibody 83606-5-RRDownload protocol
IF protocol for PI3 Kinase p85 Beta antibody 83606-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols