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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PILRA Polyclonal antibody

PILRA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11818-1-AP

Synonyms

Cell surface receptor FDF03, FDF03, Inhibitory receptor PILR alpha, PILRA, PILRα

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PILRA Polyclonal antibody (11818-1-AP)

    WB analysis using 11818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11818-1-AP (PILRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11818-1-AP targets PILRA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PILRA fusion protein Ag2385 相同性解析による交差性が予測される生物種
Full Name paired immunoglobin-like type 2 receptor alpha
Calculated molecular weight 303 aa, 34 kDa
Observed molecular weight50 kDa
GenBank accession numberBC017812
Gene symbol PILRA
Gene ID (NCBI) 29992
RRIDAB_2163648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. Paired immunoglobulin-like type 2 receptor alpha (PILRα) and PILRβ are paired receptors bearing highly similar extracellular ligand-binding domains but divergent intracellular signaling domains. PILRα is the immune inhibitory receptor possessing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain enabling it to deliver inhibitory signals. Binding of PILRα to its ligand CD99 is involved in immune regulation. PILRα can also recognize other ligands, PILR-associating neural protein (PANP), and HSV-1 glycoprotein B (HSV-1 gB). PILRα has a calculated molecular weight of 34 kDa. It has been reported that PILRα migrates at 44-50 kDa due to glycosylation (PMID: 10903717; 10660620). The other band of about 61 kDa detected by this antibody may present a heavily glycosylated form of PILRα.

Protocols

Product Specific Protocols
WB protocol for PILRA antibody 11818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols