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  • Featured Product
  • KD/KO Validated

PIN1 Recombinant antibody

PIN1 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5N20

Cat no : 81857-1-RR

Synonyms

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8, 5N20

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIN1 Recombinant antibody (81857-1-RR)

    WB analysis of HeLa using 81857-1-RR

    WB result of PIN1 antibody (81857-1-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PIN1 Recombinant antibody (81857-1-RR)

    WB analysis using 81857-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81857-1-RR (PIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PIN1 Recombinant antibody (81857-1-RR)

    IP experiment of HepG2 using 81857-1-RR

    IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with HepG2 cells lysate 1920 ug.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PIN1 Recombinant antibody (81857-1-RR)

    IP experiment of NIH/3T3 using 81857-1-RR

    IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with NIH/3T3 cells lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PIN1 Recombinant antibody (81857-1-RR)

    IHC staining of human pancreas cancer using 81857-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PIN1 Recombinant antibody (81857-1-RR)

    IHC staining of human pancreas cancer using 81857-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PIN1 Recombinant antibody (81857-1-RR)

    IF Staining of NIH/3T3 using 81857-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (81857-1-RR, Clone: 5N20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81857-1-RR

    Affinity and Kinetic Characterization of 81857-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81857-1-RR against Human PIN1 were performed. The affinity constant is 0.17 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IP detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81857-1-RR targets PIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIN1 fusion protein Ag0767 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC002899
Gene symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Protocols

Product Specific Protocols
WB protocol for PIN1 antibody 81857-1-RRDownload protocol
IHC protocol for PIN1 antibody 81857-1-RRDownload protocol
IF protocol for PIN1 antibody 81857-1-RRDownload protocol
IP protocol for PIN1 antibody 81857-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols