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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of mouse liver using 15789-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of mouse liver using 15789-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of HeLa using 15789-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of HeLa using 15789-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of mouse brain using 15789-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PIN4 Polyclonal antibody (15789-1-AP)

    WB analysis of mouse kidney using 15789-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human liver cancer using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human liver cancer using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human kidney using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human kidney using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human liver using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PIN4 Polyclonal antibody (15789-1-AP)

    IHC staining of human liver using 15789-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIN4 Polyclonal antibody (15789-1-AP)

    IF Staining of HepG2 using 15789-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIN4 antibody (15789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  • Featured Product
  • KD/KO Validated

PIN4 Polyclonal antibody

PIN4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15789-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Peptidyl-prolyl cis/trans isomerase EPVH, Parvulin-17, Parvulin-14, hPar14, EPVH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15789-1-AP targets PIN4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN4 fusion protein Ag8489 相同性解析による交差性が予測される生物種
Full Name protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
Calculated molecular weight 131 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005234
Gene Symbol PIN4
Gene ID (NCBI) 5303
RRIDAB_2878186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIN4, also known as EPVH, hPar14, is a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. PIN4 is the first eukaryotic member of the parvulin family of PPIases and contains a PPIase domain preceded by a 40-amino acid glycine- and lysine-rich N-terminal sequence.

Protocols

Product Specific Protocols
WB protocol for PIN4 antibody 15789-1-APDownload protocol
IHC protocol for PIN4 antibody 15789-1-APDownload protocol
IF protocol for PIN4 antibody 15789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation

Authors - Miki Naito
  • KD Validated
View Article