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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PINK1 Recombinant antibody, PBS Only (Capture)

PINK1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240449E7

Cat no : 81991-4-PBS

Synonyms

PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PINK1 Recombinant antibody (81991-4-RR)

    WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using PINK1 Recombinant antibody (81991-4-RR)

    FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00404-3

    Cytometric bead array standard curve of MP00404-3

    Cytometric bead array standard curve of MP00404-3, PINK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81991-4-PBS. Detection antibody: 81991-2-PBS. Standard: Ag19825. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 81991-4-RR

    Affinity and Kinetic Characterization of 81991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81991-4-PBS targets PINK1 as part of a matched antibody pair:

MP00404-3: 81991-4-PBS capture and 81991-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PINK1 fusion protein Ag19825 相同性解析による交差性が予測される生物種
Full Name PTEN induced putative kinase 1
Calculated molecular weight 581 aa, 63 kDa
GenBank accession numberBC028215
Gene symbol PINK1
Gene ID (NCBI) 65018
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.