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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human skin using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human skin using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GCDFP-15/PIP Polyclonal antibody (16068-1-AP)

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GCDFP-15/PIP Polyclonal antibody

GCDFP-15/PIP Polyclonal Antibody for IHC, ELISA
Cat No. 16068-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human skin tissue, human breast tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16068-1-AP targets GCDFP-15/PIP in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9004

Product name: Recombinant human GCDFP-15, PIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-146 aa of BC010950

Sequence: AQDNTRKIIIKNFDIPKSVRPNDEVTAVLAVQTELKECMVVKTYLISSIPLQGAFNYKYTACLCDDNPKTFYWDFYTNRTVQIAAVVDVIRELGICPDDAAVIPIKNNRFYTIEILKVE

相同性解析による交差性が予測される生物種
Full Name prolactin-induced protein
Calculated molecular weight 146 aa, 17 kDa
GenBank accession numberBC010950
Gene Symbol GCDFP-15
Gene ID (NCBI) 5304
RRIDAB_2878212
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCDFP-15 (gross cystic disease fluid protein 15), also known as PIP (prolactin-induced protein) is a secretory glycoprotein expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands (PMID: 12393800). GCDFP-15 expression is increased by prolactin and androgen and inhibited by estrogen (PMID: 18854942). The expression is also regulated by interleukins. GCDFP-15 is a marker of apocrine differentiation and is frequently used for assessment of metastases or regional recurrences of breast origin.

Protocols

Product Specific Protocols
IHC protocol for GCDFP-15/PIP antibody 16068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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