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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIP5K1A Monoclonal antibody, PBS Only

PIP5K1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E5G12

Cat no : 67483-1-PBS

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67483-1-PBS targets PIP5K1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PIP5K1A fusion protein Ag8355 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated molecular weight 56 kDa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC007833
Gene symbol PIP5K1A
Gene ID (NCBI) 8394
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.