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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIP5K1A Monoclonal antibody, PBS Only

PIP5K1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E5G12

Cat no : 67483-1-PBS

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PIP5K1A Monoclonal antibody (67483-1-Ig)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PIP5K1A Monoclonal antibody (67483-1-Ig)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67483-1-PBS targets PIP5K1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PIP5K1A fusion protein Ag8355 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated molecular weight 56 kDa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC007833
Gene symbol PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2882710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.