• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PIR Polyclonal antibody

PIR Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10263-1-AP

Synonyms

Probable quercetinase, Probable quercetin 2,3-dioxygenase PIR, Pirin, EC:1.13.11.24

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using PIR Polyclonal antibody (10263-1-AP)

    WB analysis of HepG2 using 10263-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIR Polyclonal antibody (10263-1-AP)

    WB analysis of HeLa using 10263-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PIR Polyclonal antibody (10263-1-AP)

    WB analysis of HeLa using 10263-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PIR Polyclonal antibody (10263-1-AP)

    IP experiment of HeLa using 10263-1-AP

    IP result of anti-PIR (IP:10263-1-AP, 4ug; Detection:10263-1-AP 1:300) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PIR Polyclonal antibody (10263-1-AP)

    IHC staining of human stomach using 10263-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10263-1-AP (PIR antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10263-1-AP targets PIR in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIR fusion protein Ag0330 相同性解析による交差性が予測される生物種
Full Name pirin (iron-binding nuclear protein)
Calculated molecular weight 32 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002517
Gene symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2283879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

Protocols

Product Specific Protocols
WB protocol for PIR antibody 10263-1-APDownload protocol
IHC protocol for PIR antibody 10263-1-APDownload protocol
IP protocol for PIR antibody 10263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

PIR promotes tumorigenesis of breast cancer by upregulating cell cycle activator E2F1.

Authors - Muhammad Suleman
  • KD Validated
View Article