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Validation Data Gallery

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  • Western Blot (WB) analysis of HSC-T6 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of HSC-T6 using 67516-1-Ig (same clone as 67516-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of NIH/3T3 using 67516-1-Ig (same clone as 67516-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of HeLa using 67516-1-Ig (same clone as 67516-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using PIR Monoclonal antibody (67516-1-Ig)

    WB analysis of K-562 using 67516-1-Ig (same clone as 67516-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67516-1-PBS in a different storage buffer formulation.

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PIR Monoclonal antibody, PBS Only

PIR Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67516-1-PBS
Clone No.2A10E4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

PIR, Pirin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67516-1-PBS targets PIR in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PIR fusion protein Ag29969 相同性解析による交差性が予測される生物種
Full Name pirin (iron-binding nuclear protein)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002517
Gene Symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2882737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00625
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).