Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
20990-1-AP targets PIRT in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoinositide-interacting regulator of transient receptor potential channels |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | NM_001101387 |
| Gene Symbol | PIRT |
| Gene ID (NCBI) | 644139 |
| RRID | AB_2878785 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0C851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIRT is a key component of the TRPV1 complex that positively regulates TRPV1 activity. It binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI). PIRT is a membrane protein with MW 15-25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIRT antibody 20990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep Defective branched-chain amino acid catabolism in dorsal root ganglia contributes to mechanical pain | ||
Evid Based Complement Alternat Med C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4. |