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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PISD Monoclonal antibody

PISD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B6C1

Cat no : 68484-1-Ig

Synonyms

DJ858B16, dJ858B16.2, DKFZp566G2246, PISD, PSDC, PSSC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PISD Monoclonal antibody (68484-1-Ig)

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PISD Monoclonal antibody (68484-1-Ig)

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68484-1-Ig targets PISD in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PISD fusion protein Ag9499 相同性解析による交差性が予測される生物種
Full Name phosphatidylserine decarboxylase
Calculated molecular weight375aa,43 kDa; 408aa,46 kDa
Observed molecular weight46 kDa
GenBank accession numberBC001482
Gene symbol PISD
Gene ID (NCBI) 23761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PISD antibody 68484-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols