Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human placenta using 10101-2-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10101-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10101-2-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10101-2-AP (PITRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 10101-2-AP
IP result of anti-PITRM1 (IP:10101-2-AP, 4ug; Detection:10101-2-AP 1:500) with A549 cells lysate 2000ug.
IHC staining of human breast cancer using 10101-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10101-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human osteosarcoma using 10101-2-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human osteosarcoma using 10101-2-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 10101-2-AP (PITRM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 10101-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PITRM1 antibody (10101-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PITRM1 Polyclonal antibody

PITRM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10101-2-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MP1, Metalloprotease 1, KIAA1104, hPreP, hMP1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human placenta tissue, A549 cells, mouse testis tissue
Positive IP detected in	A549 cells
Positive IHC detected in	human breast cancer tissue, human osteosarcoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

10101-2-AP targets PITRM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PITRM1 fusion protein Ag0150 相同性解析による交差性が予測される生物種
Full Name	pitrilysin metallopeptidase 1
Calculated molecular weight	60 kDa, 117 kDa
Observed molecular weight	110-117 kDa
GenBank accession number	BC001150
Gene Symbol	PITRM1
Gene ID (NCBI)	10531
RRID	AB_2165145
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5JRX3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.This antibody is specific to PITRM1.

Protocols

Product Specific Protocols
WB protocol for PITRM1 antibody 10101-2-AP	Download protocol
IHC protocol for PITRM1 antibody 10101-2-AP	Download protocol
IF protocol for PITRM1 antibody 10101-2-AP	Download protocol
IP protocol for PITRM1 antibody 10101-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun DELE1 tracks perturbed protein import and processing in human mitochondria. Authors - Evelyn Fessler View Article
mouse	WB	PLoS One Proteolysis controls endogenous substance p levels. Authors - Mitchell Andrew J AJ View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 10101-2-AP

WB analysis of A549 using 10101-2-AP

WB analysis of mouse testis using 10101-2-AP

IP experiment of A549 using 10101-2-AP

IHC staining of human breast cancer using 10101-2-AP