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  • Featured Product
  • KD/KO Validated

PITX2 Monoclonal antibody, PBS Only

PITX2 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1E3B1

Cat no : 67201-1-PBS

Synonyms

RIEG bicoid-related homeobox transcription factor, Paired-like homeodomain transcription factor 2, ARP1, ALL1-responsive protein ARP1, 1E3B1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PITX2 Monoclonal antibody (67201-1-Ig)

    WB analysis using 67201-1-Ig (same clone as 67201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67201-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PITX2 Monoclonal antibody (67201-1-Ig)

    WB analysis of HEK-293 using 67201-1-Ig (same clone as 67201-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67201-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67201-1-PBS targets PITX2 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PITX2 fusion protein Ag27924 相同性解析による交差性が予測される生物種
Full Name paired-like homeodomain 2
Calculated molecular weight 36 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013998
Gene symbol PITX2
Gene ID (NCBI) 5308
RRIDAB_2882494
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PITX2 belongs to the bicoid class of homeodomain transcription factors, which play essential roles in embryonic development. Various PITX2 isoforms are involved in a wide variety of developmental programs, including left-right signaling. It controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, PITX2 is involved in the expansion of muscle progenitors. In addition, it may play a role in the proper localization of asymmetric organs such as the heart and stomach. PITX2 can be bound by some other proteins, and are part of a much larger atrial transcriptional complex. This antibody specifically recognizes isoform PTX2C.