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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIWIL1 Polyclonal antibody, PBS Only

PIWIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15659-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    WB analysis of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    WB analysis of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    WB analysis of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IP experiment of mouse testis using 15659-1-AP (same clone as 15659-1-PBS)

    IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:2000) with mouse testis tissue lysate 2400 ug. This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human liver cancer using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human liver cancer using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human prostate cancer using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human prostate cancer using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PIWIL1 Polyclonal antibody (15659-1-AP)

    IHC staining of human testis using 15659-1-AP (same clone as 15659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PIWIL1 Polyclonal antibody (15659-1-AP)

    IF Staining of HepG2 using 15659-1-AP (same clone as 15659-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15659-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15659-1-PBS targets PIWIL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIWIL1 fusion protein Ag8056 相同性解析による交差性が予測される生物種
Full Name piwi-like 1 (Drosophila)
Calculated molecular weight 99 kDa
Observed molecular weight 85-95 kDa
GenBank accession numberBC028581
Gene symbol PIWIL1
Gene ID (NCBI) 9271
RRIDAB_2878163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.