• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PJA1 Polyclonal antibody, PBS Only

PJA1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17687-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of rat brain using 17687-1-AP (same clone as 17687-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PJA1 Polyclonal antibody (17687-1-AP)

    IP experiment of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

    IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17687-1-PBS targets PJA1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PJA1 fusion protein Ag11820 相同性解析による交差性が予測される生物種
Full Name praja ring finger 1
Calculated molecular weight 643 aa, 71 kDa
Observed molecular weight 71-80 kDa
GenBank accession numberBC075803
Gene symbol PJA1
Gene ID (NCBI) 64219
RRIDAB_2252483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.