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  • KD/KO Validated

PKM2-specific Monoclonal antibody

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, ICC

Conjugate

Unconjugated

CloneNo.

1C11C7

Cat no : 60268-1-Ig

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK


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Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60268-1-Ig targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA, ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_002654
Gene symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2881388
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IHC protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IF protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IP protocol for PKM2-specific antibody 60268-1-IgDownload protocol
FC protocol for PKM2-specific antibody 60268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Mater

Dual-Targeted Novel Temozolomide Nanocapsules Encapsulating siPKM2 Inhibit Aerobic Glycolysis to Sensitize Glioblastoma to Chemotherapy

Authors - Yongkang Zhang
mouse,pigWB

Cell Metab

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Authors - Xiaolei Lin
humanWB,IP,IHC,IF

Nat Commun

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Authors - Jianxiong Chen
humanWB,IF

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
ratWB,IF,ICC

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
  • KD Validated
humanWB

Autophagy

SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.

Authors - Bin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Ovarian tissue
PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:60268-1-Ig)