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PKM2-specific Monoclonal antibody

Name: PKM2-specific Monoclonal antibody
Brand: Proteintech
SKU: 60268-1-Ig
Rating: 5.00 (1 reviews)

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, ICC

Conjugate

Unconjugated

Publications

CloneNo.

1C11C7

Reviews (1)

Cat no : 60268-1-Ig

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 60268-1-Ig
WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells.
WB analysis using 60268-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of MCF-7 using 60268-1-Ig
Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 60268-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 60268-1-Ig
Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 60268-1-Ig
C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 60268-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 60268-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 60268-1-Ig
IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug.
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 10x lens).
IHC staining of human colon cancer using 60268-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 40x lens).
IF Staining of HeLa using 60268-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 60268-1-Ig
Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

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Cat No. 60268-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:30000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 49 publications below
IHC	See 9 publications below
IF	See 20 publications below
IP	See 7 publications below
CoIP	See 5 publications below

Product Information

60268-1-Ig targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA, ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	PKM pyruvate kinase, muscle
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	NM_002654
Gene symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_2881388
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM2-specific antibody 60268-1-Ig	Download protocol
IHC protocol for PKM2-specific antibody 60268-1-Ig	Download protocol
IF protocol for PKM2-specific antibody 60268-1-Ig	Download protocol
IP protocol for PKM2-specific antibody 60268-1-Ig	Download protocol
FC protocol for PKM2-specific antibody 60268-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Mater Dual-Targeted Novel Temozolomide Nanocapsules Encapsulating siPKM2 Inhibit Aerobic Glycolysis to Sensitize Glioblastoma to Chemotherapy Authors - Yongkang Zhang View Article
mouse,pig	WB	Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation Authors - Xiaolei Lin View Article
human	WB,IP,IHC,IF	Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration Authors - Jianxiong Chen View Article
human	WB,IF	Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. Authors - Youming Zhu View Article
rat	WB,IF,ICC	Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. Authors - Xunxun Wu KD Validated View Article
human	WB	Autophagy SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect. Authors - Bin Zhang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.

Applications: Immunofluorescence
Primary Antibody Dilution: 1:200
Cell Tissue Type: Ovarian tissue

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:60268-1-Ig)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKM2-specific Monoclonal antibody

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 60268-1-Ig

WB analysis using 60268-1-Ig

WB analysis of MCF-7 using 60268-1-Ig

WB analysis of human brain using 60268-1-Ig

WB analysis of MCF-7 using 60268-1-Ig

WB analysis of C6 using 60268-1-Ig

WB analysis of NIH/3T3 using 60268-1-Ig

WB analysis of rat brain using 60268-1-Ig

IP experiment of HeLa using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IHC staining of human colon cancer using 60268-1-Ig

IF Staining of HeLa using 60268-1-Ig

IF Staining of HepG2 using 60268-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dual-Targeted Novel Temozolomide Nanocapsules Encapsulating siPKM2 Inhibit Aerobic Glycolysis to Sensitize Glioblastoma to Chemotherapy

Augmentation of scleral glycolysis promotes myopia through histone lactylation

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.

Reviews

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