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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKC Gamma Polyclonal antibody, PBS Only

PKC Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29208-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Gamma Polyclonal antibody (29208-1-AP)

    WB analysis using 29208-1-AP (same clone as 29208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29208-1-AP (PKC Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PKC Gamma Polyclonal antibody (29208-1-AP)

    IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PKC Gamma Polyclonal antibody (29208-1-AP)

    IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29208-1-PBS targets PKC Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Gamma fusion protein Ag25085 相同性解析による交差性が予測される生物種
Full Name protein kinase C, gamma
Calculated molecular weight 78 kDa
Observed molecular weight78 kDa
GenBank accession numberBC047876
Gene symbol PKC Gamma
Gene ID (NCBI) 5582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.