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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKC Gamma Monoclonal antibody, PBS Only

PKC Gamma Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

pig, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4B9

Cat no : 66429-1-PBS

Synonyms

PRKCG, PKC-gamma, PKCG, PKCC, EC:2.7.11.13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Gamma Monoclonal antibody (66429-1-Ig)

    WB analysis using 66429-1-Ig (same clone as 66429-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-Ig (PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IHC staining of human brain using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66429-1-Ig (PKC gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    IF Staining of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66429-1-Ig(PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using PKC Gamma Monoclonal antibody (66429-1-Ig)

    FC experiment of SH-SY5Y using 66429-1-Ig (same clone as 66429-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (66429-1-Ig, Clone:2F4B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66429-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66429-1-PBS targets PKC Gamma in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Reactivity pig, rat, mouse, human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PKC Gamma fusion protein Ag5910 相同性解析による交差性が予測される生物種
Full Name protein kinase C, gamma
Calculated molecular weight 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC047876
Gene symbol PKC Gamma
Gene ID (NCBI) 5582
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.