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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis using 66493-1-Ig (same clone as 66493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of MCF-7 using 66493-1-Ig (same clone as 66493-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of HEK-293 using 66493-1-Ig (same clone as 66493-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of ROS1728 using 66493-1-Ig (same clone as 66493-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    WB analysis of C6 using 66493-1-Ig (same clone as 66493-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IHC staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of human lung cancer using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Iota Monoclonal antibody (66493-1-Ig)

    IF Staining of HeLa using 66493-1-Ig (same clone as 66493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66493-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PKC Iota Monoclonal antibody, PBS Only

PKC Iota Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66493-1-PBS
Clone No.3A1B6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

PRKCI, EC:2.7.11.13, DXS1179E, Atypical protein kinase C-lambda/iota, aPKC-lambda/iota

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66493-1-PBS targets PKC Iota in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PKC Iota fusion protein Ag4990 相同性解析による交差性が予測される生物種
Full Name protein kinase C, iota
Calculated molecular weight 68 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC022016
Gene Symbol PKC Iota
Gene ID (NCBI) 5584
RRIDAB_2881858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41743
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.