PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26899-1-AP

Synonyms

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13


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Tested Applications

Positive WB detected inHEK-293T cells, HT-29 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26899-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Zeta fusion protein Ag25391 相同性解析による交差性が予測される生物種
Full Name protein kinase C, zeta
Calculated molecular weight 80 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC014270
Gene symbol PKC Zeta
Gene ID (NCBI) 5590
RRIDAB_2880675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.

Protocols

Product Specific Protocols
WB protocol for PKC Zeta antibody 26899-1-APDownload protocol
IHC protocol for PKC Zeta antibody 26899-1-APDownload protocol
IF protocol for PKC Zeta antibody 26899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

HRS plays an important role for TLR7 signaling to orchestrate inflammation and innate immunity upon EV71 infection.

Authors - Zhen Luo
mouseWB

JCI Insight

Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.

Authors - Hongyi Zhou
WB

Fish Shellfish Immunol

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Authors - Zhong-Wen Chang
mouseWB

J Cell Mol Med

Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis.

Authors - Qianxia Yin
humanWB

Cell Rep Med

Arachidonic acid released by PIK3CA mutant tumor cells triggers malignant transformation of colonic epithelium by inducing chromatin remodeling

Authors - Baoyu He
  • KD Validated
humanIF

Dev Cell

Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation

Authors - Hiroto Kinoshita