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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKLR Polyclonal antibody

PKLR Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17580-1-AP

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PKLR Polyclonal antibody (17580-1-AP)

    WB analysis of K-562 using 17580-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PKLR Polyclonal antibody (17580-1-AP)

    WB analysis of NIH/3T3 using 17580-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PKLR Polyclonal antibody (17580-1-AP)

    WB analysis of mouse liver using 17580-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PKLR Polyclonal antibody (17580-1-AP)

    WB analysis of mouse kidney using 17580-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PKLR Polyclonal antibody (17580-1-AP)

    IP experiment of K-562 using 17580-1-AP

    IP result of anti-PKLR (IP:17580-1-AP, 4ug; Detection:17580-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using PKLR Polyclonal antibody (17580-1-AP)

    IHC staining of human liver using 17580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PKLR Polyclonal antibody (17580-1-AP)

    IHC staining of human liver using 17580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17580-1-AP targets PKLR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKLR fusion protein Ag11096 相同性解析による交差性が予測される生物種
Full Name pyruvate kinase, liver and RBC
Calculated molecular weight 574 aa, 62 kDa, 585 aa, 63 kDa
Observed molecular weight 58-62 kDa
GenBank accession numberBC025737
Gene symbol PKLR
Gene ID (NCBI) 5313
RRIDAB_2268201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Protocols

Product Specific Protocols
WB protocol for PKLR antibody 17580-1-APDownload protocol
IHC protocol for PKLR antibody 17580-1-APDownload protocol
IP protocol for PKLR antibody 17580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols