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PKLR Polyclonal antibody

Name: PKLR Polyclonal antibody
Brand: Proteintech
SKU: 22456-1-AP

PKLR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22456-1-AP

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

Validation Data Gallery

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AllWBIHC

WB analysis of multi-cells/tissue using 22456-1-AP
WB results of 22456-1-AP.
WB analysis using 22456-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2.MCF7 cells using 22456-1-AP
WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).
WB analysis using 22456-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle tissue using 22456-1-AP
WB result of 22456-1-AP. (anti-PKM2 and PKLR antibodies are used as negative controls.).
WB analysis of human kidney using 22456-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 22456-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22456-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22456-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse muscle/liver tissue using 22456-1-AP
WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).
WB analysis of mouse heart tissue using 22456-1-AP
WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).
WB analysis of 293 cell using 22456-1-AP
WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).
WB analysis of HeLa using 22456-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22456-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 22456-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22456-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22456-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 22456-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 22456-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissue, mouse heart tissue, 293 cell, HepG2.MCF7 cells, HeLa cells, MCF-7 cells, human kidney tissue, human liver tissue, NIH/3T3 cells, HEK-293 cells, Raji cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 15 publications below
IHC	See 3 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

22456-1-AP targets PKLR in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PKLR fusion protein Ag17933 相同性解析による交差性が予測される生物種
Full Name	pyruvate kinase, liver and RBC
Calculated molecular weight	574 aa, 62 kDa
Observed molecular weight	58-62 kDa
GenBank accession number	BC025737
Gene symbol	PKLR
Gene ID (NCBI)	5313
RRID	AB_10918271
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Protocols

Product Specific Protocols
WB protocol for PKLR antibody 22456-1-AP	Download protocol
IHC protocol for PKLR antibody 22456-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Med Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction. Authors - Weier Qi View Article
rat	WB	Cell Metab Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health. Authors - Abudukadier Abulizi View Article
human	WB,IHC	Mol Ther Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis. Authors - Zhijun Liu View Article
mouse	WB	PLoS Genet PMID: 24831725 Authors - Yukimasa Takeda View Article
mouse	WB	Int J Mol Sci Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice. Authors - Yong-Hui Jiang View Article
human	WB,IHC	Sci Rep Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer. Authors - Wei Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKLR Polyclonal antibody

PKLR Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of multi-cells/tissue using 22456-1-AP

WB analysis using 22456-1-AP

WB analysis of HepG2.MCF7 cells using 22456-1-AP

WB analysis using 22456-1-AP

WB analysis of mouse skeletal muscle tissue using 22456-1-AP

WB analysis of human kidney using 22456-1-AP

WB analysis of human liver using 22456-1-AP

WB analysis of NIH/3T3 using 22456-1-AP

WB analysis of HEK-293 using 22456-1-AP

WB analysis of mouse muscle/liver tissue using 22456-1-AP

WB analysis of mouse heart tissue using 22456-1-AP

WB analysis of 293 cell using 22456-1-AP

WB analysis of HeLa using 22456-1-AP

WB analysis of MCF-7 using 22456-1-AP

IHC staining of human lung cancer using 22456-1-AP

IHC staining of human lung cancer using 22456-1-AP

IHC staining of human breast cancer using 22456-1-AP

IHC staining of human breast cancer using 22456-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.

Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.

Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis.

PMID: 24831725

Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice.

Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.