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Validation Data Gallery

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  • Western Blot (WB) analysis of KEK293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of KEK293 cells using 25659-1-AP (same clone as 25659-1-PBS)

    WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM Polyclonal antibody (25659-1-AP)

    WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of HEK-293 using 25659-1-AP (same clone as 25659-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of MCF-7 using 25659-1-AP (same clone as 25659-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of COLO 320 using 25659-1-AP (same clone as 25659-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using PKM Polyclonal antibody (25659-1-AP)

    WB analysis of SMMC-7721 using 25659-1-AP (same clone as 25659-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PKM Polyclonal antibody (25659-1-AP)

    IHC staining of human colon using 25659-1-AP (same clone as 25659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKM Polyclonal antibody (25659-1-AP)

    IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PKM Polyclonal antibody, PBS Only

PKM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25659-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25659-1-PBS targets PKM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKM fusion protein Ag20270 相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC000481
Gene Symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2880181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14618
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.