Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of 293 cell using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of HepG2/MCF7 cells using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of multi-cells/tissue using 15821-1-AP (same clone as 15821-1-PBS)
WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse muscle/liver tissues using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart tissue using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)
IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PKM1-specific Polyclonal antibody, PBS Only

PKM1-specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15821-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PKM, Pyruvate kinase PKM, OIP 3, EC:2.7.11.1, EC:2.7.10.2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15821-1-PBS targets PKM1-specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	PKM pyruvate kinase, muscle
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	NM_182470
Gene Symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_2163820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14618
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of mouse skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of 293 cell using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of HepG2/MCF7 cells using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of multi-cells/tissue using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of mouse muscle/liver tissues using 15821-1-AP (same clone as 15821-1-PBS)

WB analysis of mouse heart tissue using 15821-1-AP (same clone as 15821-1-PBS)

IP experiment of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

PKM1-specific Polyclonal antibody, PBS Only

PKM1-specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information