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  • Featured Product
  • KD/KO Validated

PKM1-specific Polyclonal antibody, PBS Only

PKM1-specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15821-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of multi-cells/tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of multi-cells/tissue using 15821-1-AP (same clone as 15821-1-PBS)

    WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse muscle/liver tissues using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of mouse muscle/liver tissues using 15821-1-AP (same clone as 15821-1-PBS)

    WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of mouse heart tissue using 15821-1-AP (same clone as 15821-1-PBS)

    WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 293 cell using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of 293 cell using 15821-1-AP (same clone as 15821-1-PBS)

    WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2/MCF7 cells using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of HepG2/MCF7 cells using 15821-1-AP (same clone as 15821-1-PBS)

    WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of mouse skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PKM1-specific Polyclonal antibody (15821-1-AP)

    WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

    rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IP experiment of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

    IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PKM1-specific Polyclonal antibody (15821-1-AP)

    IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15821-1-PBS targets PKM1-specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_182470
Gene symbol PKM
Gene ID (NCBI) 5315
RRIDAB_2163820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.