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PKM Polyclonal antibody

Name: PKM Polyclonal antibody
Brand: Proteintech
SKU: 10078-2-AP

PKM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10078-2-AP

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2

Validation Data Gallery

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AllWBIHCIF/ICCFC (Intra)

WB analysis of HEK-293 cells using 10078-2-AP
WB result of PKM antibody (10078-2-AP, 1:1000) with si-Control and si-PKM transfected HEK293 cells.
WB analysis of multi-cells/tissue using 10078-2-AP
WB result of 10078-2-AP (PKM antibody) with various lysates.
WB analysis using 10078-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat skeletal muscle using 10078-2-AP
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10078-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10078-2-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10078-2-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10078-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10078-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10078-2-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10078-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 10078-2-AP
Raji cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10078-2-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10078-2-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10078-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10078-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10078-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10078-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10078-2-AP (PKM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10078-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10078-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10078-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (10078-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 10078-2-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKM (10078-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10078-2-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, multi-cells/tissue, Raji cells, SH-SY5Y cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, NIH/3T3 cells, HepG2 cells, HEK-293 cells, rat skeletal muscle tissue, mouse brain tissue, mouse muscle tissue, mouse heart tissue
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 21 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

10078-2-AP targets PKM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PKM fusion protein Ag0110 相同性解析による交差性が予測される生物種
Full Name	PKM pyruvate kinase, muscle
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	BC000481
Gene symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_2283775
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

Protocols

Product Specific Protocols
WB protocol for PKM antibody 10078-2-AP	Download protocol
IHC protocol for PKM antibody 10078-2-AP	Download protocol
IF protocol for PKM antibody 10078-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. Authors - Sefan Asamitsu View Article
pig	WB	Br J Pharmacol PMID: 30515768 Authors - Xiaoying Lian View Article
human	WB	Oncotarget CDC5L drives FAH expression to promote metabolic reprogramming in melanoma. Authors - Zhichao Gu View Article
human	WB,IP	J Cell Mol Med METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination Authors - Hao Wu View Article
pig	WB	Sci Rep Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs. Authors - Liangliang Fu View Article
human	IF	Int J Biol Sci Dissection of Targeting Molecular Mechanisms of Aristolochic Acid-induced Nephrotoxicity via a Combined Deconvolution Strategy of Chemoproteomics and Metabolomics. Authors - Qian Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKM Polyclonal antibody

PKM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of HEK-293 cells using 10078-2-AP

WB analysis of multi-cells/tissue using 10078-2-AP

WB analysis using 10078-2-AP

WB analysis of rat skeletal muscle using 10078-2-AP

WB analysis of HeLa using 10078-2-AP

WB analysis of SKOV-3 using 10078-2-AP

WB analysis of NIH/3T3 using 10078-2-AP

WB analysis of SH-SY5Y using 10078-2-AP

WB analysis of Jurkat using 10078-2-AP

WB analysis of MCF-7 using 10078-2-AP

WB analysis of HepG2 using 10078-2-AP

WB analysis of Raji using 10078-2-AP

WB analysis of SH-SY5Y using 10078-2-AP

WB analysis of HEK-293 using 10078-2-AP

WB analysis of HeLa using 10078-2-AP

WB analysis of HepG2 using 10078-2-AP

WB analysis using 10078-2-AP

IHC staining of human colon cancer using 10078-2-AP

IHC staining of human colon cancer using 10078-2-AP

IHC staining of human colon cancer using 10078-2-AP

IF Staining of HeLa using 10078-2-AP

FC experiment of HepG2 using 10078-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

PMID: 30515768

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Dissection of Targeting Molecular Mechanisms of Aristolochic Acid-induced Nephrotoxicity via a Combined Deconvolution Strategy of Chemoproteomics and Metabolomics.