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  • KD/KO Validated

PKM2-specific Polyclonal antibody

PKM2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15822-1-AP

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Recombinant protein, mouse brain tissue, N/A, HEK-293T cells, mouse stomach tissue
Positive IP detected inA549 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15822-1-AP targets PKM2-specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, chicken, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_002654
Gene symbol PKM
Gene ID (NCBI) 5315
RRIDAB_1851537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM2-specific antibody 15822-1-APDownload protocol
IHC protocol for PKM2-specific antibody 15822-1-APDownload protocol
IF protocol for PKM2-specific antibody 15822-1-APDownload protocol
IP protocol for PKM2-specific antibody 15822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Nature

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Authors - Christofk Heather R HR
  • KD Validated
humanWB,IP

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
humanWB

Mol Cell

A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.

Authors - Jin-Zhou Huang
human,mouseWB,IHC

Biomaterials

PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy

Authors - Lu Wang
humanIP

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB

Clin Transl Med

USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl

Authors - Yuanming He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (09-24-2023)

The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Tumor cell lines derived from multiple systems, tissues and organs。
PKM2-specific Antibody Western Blot validation (1:1000 dilution) in Tumor cell lines derived from multiple systems, tissues and organs。 (Cat no:15822-1-AP)
FH

Kristian (Verified Customer) (06-07-2021)

PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Transgenic mouse kidney (TRACK)
PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:15822-1-AP)
FH

Kishor (Verified Customer) (02-22-2019)

I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
PKM2-specific Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:15822-1-AP)