Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 cells using 15822-1-AP
WB result of PKM2 specific antibody (15822-1-AP, 1:5000) with si-control and si-PKM2 transfected HEK293 cells.
WB analysis using 15822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. Negative control: mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue.
WB analysis using 15822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15822-1-AP
WB result of PKM2-specific antibody (15822-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKM2-specific transfected MCF-7 cells.
WB analysis using 15822-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 15822-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15822-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15822-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of Recombinant protein using 15822-1-AP
Recombinant protein were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15822-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of 293 cell using 15822-1-AP
WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.).
WB analysis using 15822-1-AP
WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).
IP experiment of A549 using 15822-1-AP
IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:4000) with A549 cells lysate 920 ug.
IP experiment of MCF-7 using 15822-1-AP
IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.
IHC staining of human lung cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 40x lens).
IHC staining of human colon using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 15822-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 15822-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 15822-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PKM2-specific Polyclonal antibody

PKM2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15822-1-AP

Publications(435)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (435)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, MCF-7 cells, N/A, Recombinant protein, HEK-293T cells, mouse stomach tissue
Positive IP detected in	A549 cells, MCF-7 cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 31 publications below
WB	See 353 publications below
IHC	See 58 publications below
IF	See 68 publications below
IP	See 14 publications below
CoIP	See 12 publications below
ChIP	See 3 publications below

Product Information

15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	PKM pyruvate kinase, muscle
Calculated molecular weight	58 kDa
Observed molecular weight	58 kDa
GenBank accession number	NM_002654
Gene Symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_1851537
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14618
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform. This antibody can detect PKM2 monomer, dimer and tetramer (PMID: 36196894).

Protocols

Product Specific Protocols
IF protocol for PKM2-specific antibody 15822-1-AP	Download protocol
IHC protocol for PKM2-specific antibody 15822-1-AP	Download protocol
IP protocol for PKM2-specific antibody 15822-1-AP	Download protocol
WB protocol for PKM2-specific antibody 15822-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity Authors - Cheng Lian View Article
mouse,human	WB	Cancer Cell Intersection of immune and oncometabolic pathways drives cancer hyperprogression during immunotherapy Authors - Gaopeng Li View Article
mouse,human	WB,IHC	Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Authors - Christofk Heather R HR KD Validated View Article
mouse,human	WB	Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation Authors - Jian Zhou View Article
mouse	WB	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
mouse	WB	Bioact Mater Designed bone-targeting ROS-responsive nanoplatform for precision glycolysis inhibition in postmenopausal osteoporosis. Authors - Qihang Wu View Article

Validation Data Gallery

WB analysis of HEK-293 cells using 15822-1-AP

WB analysis using 15822-1-AP

WB analysis using 15822-1-AP

WB analysis of MCF-7 using 15822-1-AP

WB analysis using 15822-1-AP

WB analysis of C6 using 15822-1-AP

WB analysis using 15822-1-AP

WB analysis of HeLa using 15822-1-AP

WB analysis of Recombinant protein using 15822-1-AP

WB analysis of HEK-293 using 15822-1-AP

WB analysis of 293 cell using 15822-1-AP

WB analysis using 15822-1-AP

IP experiment of A549 using 15822-1-AP

IP experiment of MCF-7 using 15822-1-AP

IHC staining of human lung cancer using 15822-1-AP

IHC staining of human lung cancer using 15822-1-AP

IHC staining of human colon using 15822-1-AP

IHC staining of human lung cancer using 15822-1-AP

IHC staining of human colon cancer using 15822-1-AP

IHC staining of human colon cancer using 15822-1-AP

IHC staining of human colon cancer using 15822-1-AP

IF Staining of MCF-7 using 15822-1-AP

IF Staining of HeLa using 15822-1-AP

PKM2-specific Polyclonal antibody

PKM2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity

Intersection of immune and oncometabolic pathways drives cancer hyperprogression during immunotherapy

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Designed bone-targeting ROS-responsive nanoplatform for precision glycolysis inhibition in postmenopausal osteoporosis.

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