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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PKMYT1 Polyclonal antibody (29489-1-AP)

    WB analysis of MCF-7 using 29489-1-AP

    WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using PKMYT1 Polyclonal antibody (29489-1-AP)

    WB analysis using 29489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

PKMYT1 Polyclonal antibody

PKMYT1 Polyclonal Antibody for WB, ELISA
Cat No. 29489-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DKFZp547K1610, FLJ20093, MYT1, Myt1 kinase, PKMYT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells, A549 cells, MKN-45 cells, NCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29489-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKMYT1 fusion protein Ag30573 相同性解析による交差性が予測される生物種
Full Name protein kinase, membrane associated tyrosine/threonine 1
Calculated molecular weight 55 kDa
Observed molecular weight60-75 kDa
GenBank accession numberNM_004203
Gene Symbol PKMYT1
Gene ID (NCBI) 9088
RRIDAB_3086137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99640
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)

Protocols

Product Specific Protocols
WB protocol for PKMYT1 antibody 29489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols