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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKMYT1 Polyclonal antibody

PKMYT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55098-1-AP

Synonyms

MYT1, Myt1 kinase, PKMYT1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of mouse brain using 55098-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of HepG2 using 55098-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of HL-60 using 55098-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of HeLa using 55098-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of mouse skin using 55098-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using PKMYT1 Polyclonal antibody (55098-1-AP)

    WB analysis of A375 using 55098-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55098-1-AP (PKMYT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, A375 cells, HeLa cells, HepG2 cells, HL-60 cells, mouse skin tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55098-1-AP targets PKMYT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase, membrane associated tyrosine/threonine 1
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_004203
Gene symbol PKMYT1
Gene ID (NCBI) 9088
RRIDAB_10888636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase, membrane associated tyrosine/threonine (PKMYT1) is a member of the Wee family of protein kinases. PKMYT1 acts as a negative regulator of the cell cycle by inactivating the CDK1-cyclinB complex through phosphorylation of Tyr14/Tyr15 and preventing cell cycle entering into mitosis at the G2/M transition. In mammalian somatic cells, PKMYT1 affects the assembly of the Golgi apparatus and endoplasmic reticulum during telophase of mitosis by inhibiting cyclin activities. Abnormal expression of PKMYT1 is closely associated with multiple types of cancers. PKMYT1 protein was mainly localized in the cytoplasm. (PMID: 31695486, PMID: 32801781)

Protocols

Product Specific Protocols
WB protocol for PKMYT1 antibody 55098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Thorac Cancer

Targeting diacylglycerol kinase α impairs lung tumorigenesis by inhibiting cyclin D3

Authors - Dong Zhou
View Article