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  • Featured Product
  • KD/KO Validated

PKN2 Polyclonal antibody

PKN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14608-1-AP

Synonyms

PRKCL2, PRK2, PKN gamma, PAK2, Pak 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of HEK-293 using 14608-1-AP

    WB result of PKN2 antibody (14608-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKN2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis using 14608-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of SKOV-3 using 14608-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of HeLa using 14608-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of K-562 using 14608-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of HEK-293 using 14608-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PKN2 Polyclonal antibody (14608-1-AP)

    WB analysis of COLO 320 using 14608-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using PKN2 Polyclonal antibody (14608-1-AP)

    IP experiment of COLO 320 using 14608-1-AP

    IP result of anti-PKN2 (IP:14608-1-AP, 4ug; Detection:14608-1-AP 1:1000) with COLO 320 cells lysate 2560 ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PKN2 Polyclonal antibody (14608-1-AP)

    IHC staining of human stomach using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PKN2 Polyclonal antibody (14608-1-AP)

    IHC staining of human colon cancer using 14608-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PKN2 Polyclonal antibody (14608-1-AP)

    IF Staining of HepG2 using 14608-1-AP

    Immunofluorescent analysis of HepG2 cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKN2 Polyclonal antibody (14608-1-AP)

    IF Staining of HeLa using 14608-1-AP

    Immunofluorescent analysis of Hela cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of HepG2 cells using PKN2 Polyclonal antibody (14608-1-AP)

    FC experiment of HepG2 using 14608-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKN2 (14608-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA2780 cells, K-562 cells, HeLa cells, HEK-293 cells, COLO 320 cells, SKOV-3 cells, HEK-293T cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman stomach tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14608-1-AP targets PKN2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKN2 fusion protein Ag6164 相同性解析による交差性が予測される生物種
Full Name protein kinase N2
Calculated molecular weight 112 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC062620
Gene symbol PKN2
Gene ID (NCBI) 5586
RRIDAB_10638487
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKN2 antibody 14608-1-APDownload protocol
IHC protocol for PKN2 antibody 14608-1-APDownload protocol
IF protocol for PKN2 antibody 14608-1-APDownload protocol
IP protocol for PKN2 antibody 14608-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer

Authors - Liya Liu
View Article
pigWB,IHC

Reprod Domest Anim

miR-26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the PAK2 gene.

Authors - Maoliang Ran
View Article