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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKP1 Polyclonal antibody (22632-1-AP)

    WB analysis using 22632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22632-1-AP (PKP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PKP1 Polyclonal antibody (22632-1-AP)

    IHC staining of human skin cancer using 22632-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 22632-1-AP (PKP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PKP1 Polyclonal antibody (22632-1-AP)

    IF Staining of PC-3 using 22632-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PKP1 antibody (22632-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Plakophilin 1 Polyclonal antibody

Plakophilin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22632-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PKP1, Plakophilin-1, B6P

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHaCaT cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22632-1-AP targets Plakophilin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plakophilin 1 fusion protein Ag18153 相同性解析による交差性が予測される生物種
Full Name plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
Calculated molecular weight 747 aa, 83 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC114571
Gene Symbol PKP1
Gene ID (NCBI) 5317
RRIDAB_3085701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

Protocols

Product Specific Protocols
WB protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
IHC protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
IF protocol for Plakophilin 1 antibody 22632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols