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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Plakophilin 3 Polyclonal antibody

Plakophilin 3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18338-1-AP

Synonyms

PKP3, plakophilin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    WB analysis of K-562 using 18338-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    WB analysis of MCF-7 using 18338-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    WB analysis of A549 using 18338-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    WB analysis of HeLa using 18338-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    IP experiment of A549 using 18338-1-AP

    IP result of anti-Plakophilin 3 (IP:18338-1-AP, 4ug; Detection:18338-1-AP 1:600) with A549 cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    IHC staining of human colon using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Plakophilin 3 Polyclonal antibody (18338-1-AP)

    IHC staining of human colon using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18338-1-AP targets Plakophilin 3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plakophilin 3 fusion protein Ag13062 相同性解析による交差性が予測される生物種
Full Name plakophilin 3
Calculated molecular weight 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC000081
Gene symbol Plakophilin 3
Gene ID (NCBI) 11187
RRIDAB_2164281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IHC protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IP protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

J Ovarian Res

Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis

Authors - Yuan Li
View Article
humanIHC

Biomedicines

Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis

Authors - Yan Du
View Article
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article