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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKP4 Polyclonal antibody

PKP4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14062-1-AP

Synonyms

p0071, PKP4, plakophilin 4

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of A431 using 14062-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PKP4 Polyclonal antibody (14062-1-AP)

    WB analysis of HepG2 using 14062-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14062-1-AP (PKP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14062-1-AP targets PKP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKP4 fusion protein Ag5113 相同性解析による交差性が予測される生物種
Full Name plakophilin 4
Calculated molecular weight 132 kDa
Observed molecular weight 125-140 kDa
GenBank accession numberBC050308
Gene symbol PKP4
Gene ID (NCBI) 8502
RRIDAB_10640532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKP4 antibody 14062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols