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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLA2G1B Polyclonal antibody, PBS Only

PLA2G1B Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15843-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G1B Polyclonal antibody (15843-1-AP)

    WB analysis using 15843-1-AP (same clone as 15843-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    WB analysis of mouse spleen using 15843-1-AP (same clone as 15843-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IF Staining of mouse pancreas using 15843-1-AP (same clone as 15843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15843-1-PBS targets PLA2G1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G1B fusion protein Ag8603 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IB (pancreas)
Calculated molecular weight70aa,8 kDa; 148aa,16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC005386
Gene symbol PLA2G1B
Gene ID (NCBI) 5319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.