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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C10

Cat no : 66397-1-Ig

Synonyms

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

Validation Data Gallery

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  • Western Blot (WB) analysis of pig pancreas tissue using PLA2G1B Monoclonal antibody (66397-1-Ig)

    WB analysis of pig pancreas using 66397-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLA2G1B Monoclonal antibody (66397-1-Ig)

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLA2G1B Monoclonal antibody (66397-1-Ig)

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLA2G1B fusion protein Ag8812 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IB (pancreas)
Calculated molecular weight70aa,8 kDa; 148aa,16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC005386
Gene symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

Protocols

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols