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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of NIH/3T3 using 68133-1-Ig (same clone as 68133-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis of A549 using 68133-1-Ig (same clone as 68133-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLA2G4A Monoclonal antibody (68133-1-Ig)

    WB analysis using 68133-1-Ig (same clone as 68133-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68133-1-Ig (PLA2G4A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IHC staining of human lung cancer using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68133-1-Ig (PLA2G4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLA2G4A Monoclonal antibody (68133-1-Ig)

    IF Staining of HeLa using 68133-1-Ig (same clone as 68133-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLA2G4A antibody (68133-1-Ig, Clone: 2D10H8 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68133-1-PBS in a different storage buffer formulation.

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PLA2G4A Monoclonal antibody, PBS Only

PLA2G4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68133-1-PBS
Clone No.2D10H8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, PLA2G4, PLA2G4A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68133-1-PBS targets PLA2G4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLA2G4A fusion protein Ag32674 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Calculated molecular weight 749 aa, 85 kDa
Observed molecular weight85-100 kDa
GenBank accession numberBC114340
Gene Symbol PLA2G4A
Gene ID (NCBI) 5321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47712
Storage Buffer PBS only
Storage ConditionsStore at -80°C.