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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    WB analysis of mouse brain using 13345-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using PLA2G4D Polyclonal antibody (13345-1-AP)

    WB analysis of Transfected HEK-293 using 13345-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLA2G4D Polyclonal antibody (13345-1-AP)

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 13345-1-AP (PLA2G4D antibody) at dilution of 1:50 (under 40x lens).

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PLA2G4D Polyclonal antibody

PLA2G4D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Cpla2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13345-1-AP targets PLA2G4D in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G4D fusion protein Ag4178 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVD (cytosolic)
Calculated molecular weight 818 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC034571
Gene Symbol PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2164607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

Protocols

Product Specific Protocols
WB protocol for PLA2G4D antibody 13345-1-APDownload protocol
IHC protocol for PLA2G4D antibody 13345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols