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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLA2G4D Monoclonal antibody, PBS Only

PLA2G4D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E2A10

Cat no : 66752-1-PBS

Synonyms

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4D Monoclonal antibody (66752-1-Ig)

    WB analysis using 66752-1-Ig (same clone as 66752-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using PLA2G4D Monoclonal antibody (66752-1-Ig)

    WB analysis of Caco-2 using 66752-1-Ig (same clone as 66752-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PLA2G4D Monoclonal antibody (66752-1-Ig)

    IF Staining of HepG2 using 66752-1-Ig (same clone as 66752-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66752-1-PBS targets PLA2G4D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLA2G4D fusion protein Ag4178 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVD (cytosolic)
Calculated molecular weight 818 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC034571
Gene symbol PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2882098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).