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  • KD/KO Validated

PLA2G4E Polyclonal antibody

PLA2G4E Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18088-1-AP

Synonyms

phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens


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Tested Applications

Positive WB detected inSH-SY5Y cells, SW 1990 cells, mouse thymus tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18088-1-AP targets PLA2G4E in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G4E fusion protein Ag12808 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVE
Calculated molecular weight 868 aa, 99 kDa
Observed molecular weight 99 kDa, 57 kDa
GenBank accession numberBC101584
Gene symbol PLA2G4E
Gene ID (NCBI) 123745
RRIDAB_10859777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

Protocols

Product Specific Protocols
WB protocol for PLA2G4E antibody 18088-1-APDownload protocol
IHC protocol for PLA2G4E antibody 18088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.

Authors - Junsheng Lou
  • KD Validated
human,mouseWB,IHC,IF

Prog Neurobiol

PLA2G4E, a candidate gene for resilience in Alzheimer´s disease and a new target for dementia treatment.

Authors - Marta Pérez-González
  • KD Validated
mouseWB

FASEB J

Group IVE cytosolic phospholipase A2 limits psoriatic inflammation by mobilizing the anti-inflammatory lipid N-acylethanolamine.

Authors - Luyiyun Liang
mouseWB

Front Genet

Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain.

Authors - Wenyue Su
mouseWB

J Neuroinflammation

Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization

Authors - Haojie Zhang
mouseWB

Skin Res Technol

Establishment of a Mouse Model for Porokeratosis Due to Mevalonate Diphosphate Decarboxylase Deficiency

Authors - Kexin Peng