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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4E Polyclonal antibody (18088-1-AP)

    WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PLA2G4E Polyclonal antibody (18088-1-AP)

    WB analysis of SH-SY5Y using 18088-1-AP (same clone as 18088-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW 1990 cells using PLA2G4E Polyclonal antibody (18088-1-AP)

    WB analysis of SW 1990 using 18088-1-AP (same clone as 18088-1-PBS)

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using PLA2G4E Polyclonal antibody (18088-1-AP)

    WB analysis of mouse thymus using 18088-1-AP (same clone as 18088-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PLA2G4E Polyclonal antibody (18088-1-AP)

    IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PLA2G4E Polyclonal antibody (18088-1-AP)

    IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PLA2G4E Polyclonal antibody (18088-1-AP)

    IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PLA2G4E Polyclonal antibody (18088-1-AP)

    IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLA2G4E Polyclonal antibody (18088-1-AP)

    IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18088-1-PBS targets PLA2G4E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G4E fusion protein Ag12808 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVE
Calculated molecular weight 868 aa, 99 kDa
Observed molecular weight 99 kDa, 57 kDa
GenBank accession numberBC101584
Gene Symbol PLA2G4E
Gene ID (NCBI) 123745
RRIDAB_10859777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3MJ16
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).