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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G4E Polyclonal antibody (29990-1-AP)

    WB analysis using 29990-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29990-1-AP (PLA2G4E antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLA2G4E Polyclonal antibody (29990-1-AP)

    IF Staining of A431 using 29990-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PLA2G4E antibody (29990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PLA2G4E Polyclonal antibody

PLA2G4E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29990-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ45651, phospholipase A2, group IVE, PLA2G4E, PLA2G4E Ens

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29990-1-AP targets PLA2G4E in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G4E fusion protein Ag32314 相同性解析による交差性が予測される生物種
Full Name phospholipase A2, group IVE
Calculated molecular weight 868 aa, 99 kDa
Observed molecular weight98 kDa
GenBank accession numberBC101584
Gene Symbol PLA2G4E
Gene ID (NCBI) 123745
RRIDAB_2935494
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

Protocols

Product Specific Protocols
WB protocol for PLA2G4E antibody 29990-1-APDownload protocol
IF protocol for PLA2G4E antibody 29990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols